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KMID : 0380020070220040249
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 4 p.249 ~ p.254
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Anti-angiogenic and Anti-cell Adhesion Effects and Their Mechanism with the Extract of Camellia japonica Leaf
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Song Min-Gyu
Seo Hyo-Jin
Moon Jae-Hak
Park Keun-Hyung
Kim Jong-Deog
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Abstract
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Anti-angiogenesis and anti-cell adhesion effects were investigated with different dose of Camellia japonica leaf (CJL) extract for applying anti-cancer, anti-metastasis and anti-obesity. Cytotoxicity on HUVECs was very low at 200 ug/mL of CJL-extract. Anti-angiogenic ratio at increasing dose of 1.5 ug/mL, 3.0 ug/mL, 15 ug/mL and 30 ug/mL was showed 30.7%, 38.5%, 53.8%, and 70.0%, respectively. Also, anti-cell adhesion effect at concentration of was expressed on E-selectin by 46.7%, 66.7%, and 86.76%, on VCAM-1, 23.0%, 61.5%, and 84.6%, and on ICAM-1, 11%, 55.5%, and 88.8%, respectively. For inquiring anti-angiogenesis mechanism, when western blot was performed with different dose of CJL extract, signal molecules of VEGFR-2, -catenin and PI3-K were suppressed. As the signal transduction from VEGFR-2, -catenin and PI3-K to NF-B was interupted, angiogenesis could not be occurred causing not activated NF-kB. C. japonica leaf (CJL) is a useful herb for developing therapeutics of angiogenesis related diseases such as cancer, metastasis, rheumathioid arthritis and obesity.
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KEYWORD
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Camellia japonica leaf (CJL), angiogenesis, cell adhesion, signal molecules, anti-obesity
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